The organ-on-chip research line focuses on advancing the integration of sensing capabilities in microphysiological systems and organ-on-chip devices. We have developed an advanced set of technologies for monitoring biologically relevant parameters. For instance, leveraging the group’s expertise in bioimpedance and the critical role of cellular barriers in maintaining biological homeostasis, we have integrated microelectrodes for transepithelial electrical resistance (TEER) assessment—a key indicator of cell barrier integrity—in small cell culture chambers for organ-on-chip applications.
Currently, in collaboration with our clinical partners, we are pioneering a novel approach that utilizes nanotechnologies to study the tight junctions, at the molecular level. Tight junctions play a crucial role in barrier function, and our research is centred on developing nanostructured pillars capable of monitoring the behaviour of single ion channels at the tight junctions. This innovative and ground-breaking tool will provide a unique method for understanding the fundamental mechanisms that govern cell barrier function.
Our work on TEER monitoring has significant implications for various biomedical applications, including drug delivery, disease modelling, and tissue engineering. By providing real-time data on cell barrier integrity, our technology enables more accurate and dynamic assessments of how drugs and other substances interact with cellular barriers and permeate through them.
Related publications:
Yeste J, García-Ramírez M, Illa X, Guimerà A, Hernández C, Simó R, Villa R. A compartmentalized microfluidic chip with crisscross microgrooves and electrophysiological electrodes for modeling the blood–retinal barrier. Lab on a Chip 18 (2018) 95-105. DOI
Marrero D, Guimera A, Maes L, Villa R, Alvarez M, Illa X. Organ-on-a-chip with integrated semitransparent organic electrodes for barrier function monitoring. Lab on a Chip 23 (2023) 1825-1834. DOI
Contact: Jose Yeste